Figure 5.

Expression of MUC1 and Lewis(y) antigen of the glycans of MUC1 before and after α1,2-fucosyltransferase (α1,2-FT) gene transfection. (A) Western blots of immunoprecipitated MUC1 protein using corresponding antibodies and Lewis(y) antibody and HRP-labeled secondary antibodies. (B) Densitometric quantification of Lewis(y) in (A) and calculation of Lewis(y) expression/MUC1 (set the RMG-I cells as 100%) (n=3). ^*P<0.01 compared with RMG-I. IP, immunoprecipitation by the antibody of MUC1; WB, western blot with antibodies to MUC1 or Lewis(y). Data are presented as the means ± SD (n=3). ^*P<0.05.