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. 2017 Jun 16;40(2):447–453. doi: 10.3892/ijmm.2017.3030

Figure 4.

Figure 4

Overexpression of SOX4 promotes melanoma cell migration and invasion and upregulates the expression of MMP2, MMP9 and nuclear factor-κB (NF-κB) p65. Melanoma A375 cells were transfected with SOX4 overexpression lentivirus transfection for 72 h. (A) Real-time PCR assay for SOX4, p65, MMP2 and MMP9 mRNA expression. (B) Western blot analysis for SOX4, p65, MMP2 and MMP9 protein expression. (C) Migration and invasion assay. The invading cells were stained with 0.5% crystal violet, and counted in 5 random squares by microscopic. Each experiment was performed in triplicate. **P<0.01 compared to the control. SOX4-OE represents SOX4 overexpression.