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. 2017 Apr 28;6(1):1313091. doi: 10.1080/20013078.2017.1313091

Figure 1.

Figure 1.

Comparison between ultracentrifuge and Vn96-peptide for urinary EV isolation. (a) Schematic overview of urinary EV isolation between ultracentrifuge and the easier and shorter protocol by using the Vn96-peptide. Of the ultracentrifuge EV sample 100% was used for LC-MS/MS analysis, while for the Vn96-peptide 45% was used for LC-MS/MS analysis. Both isolation methods resulted in a comparable number of identified proteins by LC-MS/MS and total spectral counts. (b) Representative western blot (of n = 3) showing the effect of 50 and 100 µg of the Vn96-peptide and the abundancy of PDCD6IP and TSG101. UC = ultracentrifuge, unbound indicates isolated EVs from supernatant after isolation with VN96-peptide. (c) Venn diagram comparing the identified proteins in urinary EVs isolated by ultracentrifugation between our study and data available from others 15,16. (d) Venn diagram indicating the number of overlapping proteins between exosomes isolated using the ultracentrifuge and VN96-peptide. Shown proteins were identified in at least 3 of the 4 patients in each group.