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. 2017 May 16;6(1):1322454. doi: 10.1080/20013078.2017.1322454

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© 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Platelet EVs change SMC morphology. Micrographs of SMC stained with antibodies against F-actin (a), αSMA (b) or calponin (c) after control, platelet EV-, PDGF- or CXCL4-stimulation for 96 h. Nuclei were stained with DAPI. Scale bars: 50 µm. Cell surface area (µm²) (d,e) and corrected total cell fluorescence (CTCF) (f) were quantified and displayed as bar graphs or box-and-whiskers plots, respectively. p-values were calculated by ANOVA with Tukey’s (d,e) or Kruskal–Wallis (f) post-tests (n = 3).