Figure 6.

Platelet EVs increase monocytic cell adhesion and cytokine release. SMC were incubated without or with platelet EV or CD40L in the presence of indicated inhibitors (P-selectin inhibitor, eptifibatide, F1-Fk) or blocking antibody (α-CD40L). Syto13 fluorescently labelled THP-1 were perfused at 0.15 ml/min (3 dynes/cm²) and adherent cells were quantified in six different fields; n = 5–9 (a). Representative micrographs of THP-1 cells adherent to non- or platelet EV-stimulated SMC (b). SMC were incubated with indicated agonists for 24 h and IL-6 release was measured by ELISA; n = 6 (c). P-values were calculated by ANOVA with Tukey’s post-test. Scale bar: 100 µm.