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Characterisation of engineered EVs. (a) Protein isolated from the engineered EVs or the EV producing cells were detected by immunoblotting using antibodies against the various proteins shown. Cells were stably transfected with the FPLTH-Mod 199 vector (+) or empty vector (−). (b) Particle size distribution of engineered EVs as determined by nanoparticle tracking analysis. (c) Cryo-TEM image of engineered EVs collected by ultracentrifugation.
