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. 2017 Jun 14;6(1):1333882. doi: 10.1080/20013078.2017.1333882

Figure 5.

Figure 5.

Modified pre-miR-199a is preferentially loaded into TAT expressing cells. (a) Modified pre-miR-199a was measured by qRT-PCR on RNA isolated from wild type HEK293T cells (WT), empty vector expressing cells (Empty), Flag-PC94-Lamp2a vector containing modified miR-199 expressing cells (FPL Mod 199) and Flag-PC94-Lamp2a-TAT-His vector containing modified miR-199 expressing cells (FPLTH-Mod 199). Small RNA sequencing was performed on RNA isolated from FPLTH-Mod 199 or wild type EVs (b) or the producing cells (c). Data are presented as the ratios of the amount of mature miRNA in the FPLTH-Mod 199 to wild type.