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Uptake and activity of EVs in hepatocellular carcinoma (HCC) recipient cells. qRT-PCR analysis was performed to measure modified pre-miR-199a (a) and mature miR-199a-3p (b) in Sk-Hep1 and Huh7 HCC recipient cells. Cells were exposed to 2 × 1010 EVs for 24 h. (c) The expression levels of miR-199a-3p target genes were measured by qRT-PCR in the SK-Hep1 and Huh7 cells treated with EVs. Black and red bars represent cells treated with Empty and FPLTH-Mod 199 EVs, respectively.
