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. 2017 Jun 14;6(1):1333882. doi: 10.1080/20013078.2017.1333882

Table 1.

List of vector backbones that were used during the study to develop engineered HEK293T cells.

Sr. No Vectors generated to stably select HEK293T cells Description
1 Empty pEGFP-C1 backbone without Lamp2 fusion protein
2 FPLTH 3X-Flag-PC94-Lamp2a-TAT-His fusion protein
3 FPLTH-Mod 199 3X-Flag-PC94-Lamp2a-TAT-His fusion protein containing modified precursor miR-199a TAR hairpin loop
4 FPL-Mod 199 3X-Flag-PC94-Lamp2a fusion protein containing modified precursor miR-199a TAR hairpin loop
5 FPLTH-WT 199 3X-Flag-PC94-Lamp2a-TAT-His fusion protein containing wild type precursor miR-199a hairpin loop