Figure 3.

Fractionation of Nissle 1917 crude MVs by density gradient centrifugation. (a) Nissle I, II and III indicate three replicates prepared on different days. Density gradient fractionation of one crude MV preparation from UPEC 536 grown with (RF) and without (R) iron supplementation alongside three from Nissle 1917. Density gradient banding in the tubes have individual fractions bracketed and are labelled from low to high density with visible bands marked with lines. (b) Protein amount and particle count are graphed as a percentage per fraction of all recovered. Error bars are mean ± standard deviation.