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. 2017 Jun 6;6(1):1324731. doi: 10.1080/20013078.2017.1324731

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© 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Fractionation of UPEC RF crude MVs using qEV size separation columns. Three biological replicates of crude MVs isolated from UPEC 536 grown with iron supplementation (RF) were fractionated by SEC. qEV I and qEV III were performed using new qEV columns, whereas qEV II was separated on the washed qEV column after the fractionation of qEV I. Particle counts, protein, RNA and endotoxin in each fraction as a percentage of all recovered are graphed. Recovery of all fractions, relative to input and amount of molecules in the vesicle enriched fractions F8–F10 are tabulated to the side. Transmission electron microscopy of representative fractions F8–F10 are inset in the top right hand corner with two magnifications per fraction shown.