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Fractionation of E. coli crude MVs is highly reproducible when equal protein loaded is used. Three biological replicates of crude MV preparations isolated from UPEC 536 (a, b) and Nissle 1917 (c, d) grown with (RF) and without (R) iron supplementation were fractionated by SEC. Protein amount and particle counts are graphed as a percentage per fraction of all recovered. Error bars are mean ± standard deviation.
