Figure 6.

Density gradient and qEV fractionation of Mycobacterium smegmatis crude MVs. (a) Crude MV preparations from duplicate M. smegmatis cultures (MS I and MS II) were fractionated separately by either density gradient centrifugation (left) or by qEV size exclusion chromatography (right). A new qEV column was used for each size separation. (b) Protein, RNA yield, and particle counts are graphed as a percentage per fraction of all recovered. (c) PAGE profile of MV proteins with 10 µL of each fraction from MS I crude MV preparation loaded and stained with SYPRO Ruby.