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. 2017 Jun 1;7(8):2220–2230. doi: 10.7150/thno.18675

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LAMP analysis of the paper device with real-time simultaneous detection of multiple DNA targets. Target 1 (T1): 700 pg of Streptococcus.agalactiae DNA. T2: 700 pg of Streptococcus pneumoniae DNA. T3: 700 pg of Staphylococcus aureus DNA. (a) Simultaneous detection of three DNA targets in the paper device. The fluorescence of HNB in the paper device was analyzed after a 60-min LAMP reaction. Each set of data is labeled with the type of injected target DNA. (b) Real-time plot of the three targets amplified on a paper chip at 10-min intervals. (c) Real-time quantitative assay of Streptococcus pneumoniae (T2) DNA at 10-min intervals. Injected solutions contained 0.7, 7, 70, or 700 pg of Streptococcus pneumoniae genomic DNA. The amount of DNA and number of copies are indicated in the graph. (d) LAMP analysis graph for bacterial DNA extracted from clinical samples. Measurement of fluorescence in each graph was repeated three times and error bars represent standard deviation of these results.