Figure 6.

Comparison of tumor uptake of Z-hTRAIL and hTRAIL. (A) Uptake of Z-hTRAIL or hTRAIL by subcutaneous tumor grafts. Mice bearing LS174T tumor xenografts were intravenously injected with CF750-labeled Z-hTRAIL or an equal molar amount of hTRAIL followed by dynamic scanning with an optical imaging system. (B) Biodistribution of Z-hTRAIL and hTRAIL in organs/tissues. At 6 h post-injection, the mice were sacrificed, and the organs/tissues were collected and scanned. (C) Apoptosis induction mediated by Z-hTRAIL and hTRAIL. Mice bearing LS174T tumor xenografts were intravenously injected with Z-hTRAIL or an equal molar amount of hTRAIL. After 24 h, the tumor grafts were removed and sectioned under frozen conditions. The apoptotic cells were visualized using TUNEL staining (green). The nuclei of cells were visualized using DAPI (blue). Original magnification, ×200.