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. 2017 Jun 11;7(8):2339–2349. doi: 10.7150/thno.17555

Figure 1.

Figure 1

YD277 is the most potent anti-cancer compound among the tested ML264 derivatives A. HCT116, MDA-MB-231 and HCC1806 cells were treated with each compound (10 μM) for 48 h and an SRB assay was performed. DMSO and Doxorubicin were used as the negative and positive controls, respectively, and ML264 (#4) was used as the reference compound. YD277 (#12) exhibited the greatest cytotoxicity among all of the compounds tested. B. The molecular structures of ML264 and YD277. C. YD277 did not inhibit KLF5 expression in cancer cells. Three KLF5-positive cancer cell lines (HCT116, MDA-MB-468 and HCC1806) were treated with ML264 (10 μM) or YD277 (5 μM) for 24 h. The expression levels of KLF5 protein were measured by WB and GAPDH normalized quantitative data were shown below the blot. ML264 inhibited KLF5 protein expression in HCT116 and MDA-MB-468 cells.