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. 2017 Jun 11;7(8):2339–2349. doi: 10.7150/thno.17555

Figure 3.

Figure 3

YD277 induces G1 cell cycle arrest in MDA-MB-231 and MDA-MB-468 cells A. The cell cycle distributions of MDA-MB-231 and MDA-MB-468 cells after YD277 treatment. The cells were treated with YD277 at 1, 3 and 10 μM as indicated for 36 h. The cells were stained with propidium iodide (PI) for 30 min at 37°C in dark and subjected to flow cytometer analysis. ML264 was used as the control, which induced S and G2/M phase arrest. B. Compared to DMSO and ML264, YD277 (10 μM) significantly increased the percentage of MDA-MB-231 and MDA-MB-468 cells in G1 phase. Each experiment was conducted in triplicate and data are presented as the mean ± SD (n=3). *p < 0.05, **p < 0.01. C. MDA-MB-231 and MDA-MB-468 cells were treated with YD277, ML264, or DMSO for 36 h, and the expression levels of cell cycle related proteins, such as Cyclin D1, p21, and p27, were detected by WB. GAPDH normalized quantitative data were shown below each blot.