Abstract
Chlorella pyrenoidosa were allowed to photosynthesize for short periods of time in the presence of 14CO2 and HTO. Analysis of tritium and 14C labeling of photosynthetic intermediate compounds showed that the T/14C ratio of glycolic acid was comparable to that of intermediate compounds of the photosynthetic carbon reduction cycle when photosynthesis was performed in nearly 100% oxygen and only slightly higher under steady-state conditions. It is concluded that formation of labeled glycolic acid as a consequence of its proposed hydrogen transport role in photosynthesis is quantitatively of limited importance compared to the net synthesis of glycolic acid from CO2.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BASSHAM J. A., KIRK M. Dynamics of the photosynthesis of carbon compounds. I. Carboxylation reactions. Biochim Biophys Acta. 1960 Oct 7;43:447–464. doi: 10.1016/0006-3002(60)90468-6. [DOI] [PubMed] [Google Scholar]
- BASSHAM J. A., KIRK M. PHOTOSYNTHESIS OF AMINO ACIDS. Biochim Biophys Acta. 1964 Sep 4;90:553–562. doi: 10.1016/0304-4165(64)90234-x. [DOI] [PubMed] [Google Scholar]
- MOSES V., CALVIN M. Photosynthesis studies with tritiated water. Biochim Biophys Acta. 1959 Jun;33(2):297–312. doi: 10.1016/0006-3002(59)90117-9. [DOI] [PubMed] [Google Scholar]
- SIMON H., DORRER H. D., TREBST A. PHOTOSYNTHESEVERSUCHE IN TRITIUMWASSER MIT CHLORELLA. Z Naturforsch B. 1964 Aug;19:734–744. [PubMed] [Google Scholar]
- ZELITCH I. Oxidation and reduction of glycolic and glyoxylic acids in plants. II. Glyoxylic acid reductase. J Biol Chem. 1953 Apr;201(2):719–726. [PubMed] [Google Scholar]
- ZELITCH I. THE RELATION OF GLYCOLIC ACID SYNTHESIS TO THE PRIMARY PHOTOSYNTHETIC CARBOXYLATION REACTION IN LEAVES. J Biol Chem. 1965 May;240:1869–1876. [PubMed] [Google Scholar]