Figure 2.

JQ1 inhibited cell proliferation in human ATC cell lines. (ai–aiv) Four cells lines were treated with vehicle (DMSO) or with JQ1 at increasing doses of 250 nM, 500 nM, or 1000 nM for 24, 48, 72, or 96 hours. Cells were harvested, and cell numbers were counted. (bi) Immunohistochemical analysis was carried out to detect the cell proliferation marker Ki67 after cells were treated with vehicle (DMSO) or JQ1 (500 nM) for 48 hours. (bii) Quantification of Ki67-positive cells. After treatment with JQ1, the cell lines of the THJ-11T (bar 2), THJ-16T (bar 4), THJ-21T (bar 6), and THJ-29T (bar 8) cells had significantly lower numbers of cells stained for Ki67 than those treated with DMSO as controls.