Figure 8.
Bone density normalizes following recovery from CORT exposure in adolescent mice. (a) In adult mice, we observed no effect of CORT exposure on X-ray absorption in the right femur after the recovery period (P > 0.05), and (b) no difference in right femur X-ray absorption when comparing within treatment groups from week 4 to week 8 (before and after recovery) (P > 0.05). (c) In adolescent mice, X-ray absorption in the right femur normalized to that of VEH mice after the recovery period (P > 0.05). (d) When comparing within treatment groups from week 4 to week 8, we observed a robust increase in femur X-ray absorption in high CORT mice, and a decrease in low CORT mice, with a main effect of CORT dose (F2, 16 = 32.31, P < 0.0001). Post hoc Tukey tests were undertaken to probe interactions, with * indicating statistical significance between high CORT and VEH groups and # indicating statistical significance between low CORT and VEH groups (n = 4 to 8/age/treatment). Values are considered statistically significant at the P < 0.05 level.