Table 1.
Primers Used for Polymerase Chain Reaction Assays
| Gene Name | Primer Sequence | Gene Accession Number |
|---|---|---|
| Gnrh1 | F: GGGAAAGAGAAACACTGAACA | NM_008145.2 |
| R: TCTGCCATTTGATCCACCTC | ||
| Kiss1R | F: GGTGCTGGGAGACTTCATG | NM_053244.5| |
| R: ACATACCAGCGGTCCACAC | ||
| Nos1 | F: TCGATGCCAAGGCTATGTCC | NM_008712.2 |
| R: CGGACCTTGTAGCTCTTCCTC | ||
| Tac1 | F: ATGAAAATCCTCGTGGCCGT | NM_009311.2 |
| R: GTTCTGCATCGCGCTTCTTT | ||
| Tacr1 | F: GTCTGCCAAGAGCCAAGAAC | NM_009313 |
| R: CCAGCCACATCTGAGAGACA | ||
| Tacr2 | F: TCAACTTCATCTATGCCAGTCAC | NM_009314 |
| R: ATGACAGCAATAACCGCCTTG | ||
| Tac2 | F: GCTCCACAGCTTTGTCCTTC | NM_001199971.1 |
| R: GCTAGCCTTGCTCAGCACTT | ||
| Tacr3 | F: GCCATTGCAGTGGACAGGTAT | NM_021382.6 |
| R: ACGGCCTGGCATGACTTTTA | ||
| Kiss1 | F: CTCTGTGTCGCCACCTATGC | AF472576.1| |
| R: TTCCCAGGCATTAACGAGTTC | ||
| Pdyn | F: ACAGGGGGAGACTCTCATCT | NM_018863.4 |
| R: GGGGATGAATGACCTGCTTACT |
Abbreviations: F, forward; R, reverse.