Figure 2.

Fold change of PGC1α gene expression and DNA methylation. (a) qRT-CPR was performed to measure fold change of PGC1α expression in skeletal muscle sample before (Pre; open bar) and immediately after (Post; filled bar) exercise. Results are as the mean ± standard error of the mean fold change using the ΔΔCt method. *P < 0.05. (b) Correlation analysis was performed between mRNA expression of PGC1α and after primer pair 2 intensity measured via scanning PCR. PGC1α expression was positively correlated with nucleosome occupancy in the primer pair 2 region of the PGC1α promoter region, indicating repositioning of the −1 N farther upstream of the TSS in samples with higher PGC1α expression.