Table 2.

Primer Sequences for Bisulfite Sequencing and Scanning PCR

Primer Name	Primer	Sequence
PGC1α bisulfite amplification 1	Forward	5′-TAT AGT TAT TTT GTT ATG AAA TAG GGA GTT TT G-3′
	Reverse	5′-CCA ATC ACA TAA CAA AAC TAT TAA AAA ATA A-3′
PGC1α bisulfite nested amplification 2	Forward	5′-TTA TTT GTT TGT TTT GGA AGG AAA AT-3′
	Reverse	/5Biosg/AAC AAC CCT CTA CTT AAT AAA ATA ACG C-3′
PGC1α pyrosequencing primer		5′-AAG ATT GTA GGG GAT TTT GG-3′
Scanning PCR primer pair 1	Forward	5′-AGA GCA GCA GCG ACT GTA T-3′
	Reverse	5′-TAC CAG CTC CCG AAG AGT TG-3′
Scanning PCR primer pair 2	Forward	5′-CAA CTC TTC GGG AGC TGG TA-3′
	Reverse	5′-TGA GGG AGT GTT TGA AAG CG-3′
Scanning PCR primer pair 3	Forward	5′-CGC TTT CAA ACA CTC CCT CA-3′
	Reverse	5′-GCA AAG CTC CCT GTT TCA TGA C-3′
Scanning PCR primer pair 4	Forward	5′-GTC ATG AAA CAG GGA GCT TTG C-3′
	Reverse	5′-GAG GCT TCA AGC ATC ATG CT-3′