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. 2017 Apr 21;158(7):2255–2268. doi: 10.1210/en.2016-1939

Figure 5.

Figure 5.

GH induces ARv7 and IGF-1 expression in CRPC cells. Western blot analysis of AR, ARv7, and IGF-1 protein expression levels in (a) 22Rv1 cells transiently transfected with an empty vector (pIRES2-Zs-green) or with GH expression vector for 48 hours, (b) 22Rv1 cells treated with 50 ng/mL GH for 48 hours, (c) 22Rv1 cells treated for 24 hours with increasing concentrations of pegvisomant alone or (d) in combination with 50 ng/mL GH, (e) C4-2B MDVR cells treated with indicated concentrations of pegvisomant for 24 hours, (f, g) 22Rv1 cells treated for 24 hours with increasing concentrations of AZD1480 (JAK2 inhibitor) or specific STAT5 inhibitor (Millipore) in combination with 50 ng/mL GH. Experiments were repeated at least three times; representative blots are shown.