Figure 6.

RACK1-mediated PKC effect on MR genomic action. (a) M1-rMR TAT3-Gluc cells were seeded on 96-well plates. The cells were serum-starved overnight, media with DQA and with or without different concentrations of aldosterone were added, and the cells were incubated an additional 24 hours. (b) Serum-starved cells were treated with PMA (100 nM), staurosporine (10 nM), or LY333531 (10 nM) for 24 hours (*P < 0.05, **P < 0.001). (c) shRNA knockdown of PKCβ expression significantly reduced the MR transactivation. Serum-starved cells were stimulated with aldosterone 10 nM for 24 hours. The supernatants were collected and measured for reporter activity. Results are representative of at least three independent experiments. (d) PKCβ expression under PKCβ-specific shRNA by western blot.