Table 1. Macromolecule-production information for ZHD.

Source organism	C. rosea
DNA source	Genomic DNA
Forward primer†	5′-ATACCATGGACATGCGCACTCGTAGCACAA-3′
Reverse primer‡	5′-CTCGAATTCCAAAGATGCTTCTGCGTAGTT-3′
Cloning vector	pET-28b(+)
Expression vector	pET-28b(+)
Expression host	E. coli BL21 (DE3)
Complete amino-acid sequence of the construct produced§	(MD)MRTRSTISTPNGITWYYEQEGTGPDIVLVPDGLGECQMFDSSVSQIAAQGFRVTTFDMPGMSRSAKAPAETYTEVTAQKLASYVISILDALDIKHATVWGCSSGASTVVALLLGYPDRIRNAMCHELPTKLLDHLSNTAVLEDEEISNILANVMLNDVSGGSEAWQALGVEVHARLHKNYPVWARGYPRTIPPSAPVQDVEALRGKPLDWTVGAATPTESFFDNIVTATKAGVNIGLLPGMHFPYVSHPDVFAKYVVETTQKHL(WNSSSVDKLAAALEHHHHHH)

^†The NcoI site sequence is underlined.

^‡The EcoRI site sequence is underlined.

^§The artificial additional amino acids are in parentheses and the C-terminal His tag is underlined.