| Method |
Sitting-drop vapour diffusion for initial screening, hanging-drop vapour diffusion for crystal optimization |
| Plate type |
48-well plates for initial crystal screening, 24-well plates for crystal optimization |
| Temperature (K) |
277 |
| Protein concentration (mg ml−1) |
13.5 |
| Buffer composition of protein solution |
20 mM Tris–HCl pH 7.5, 30 mM NaCl, 1 mM DTT, 5%(w/v) glycerol |
| Composition of reservoir solution |
2.0 M ammonium sulfate, 8%(v/v) 2-propanol, 0.005%(w/v) agarose |
| Volume and ratio of drop |
2 µl, 1:1 ratio of protein:reservoir solution for initial crystal screening; 4 µl, 1:1 ratio of protein:reservoir solution for crystal optimization |
| Volume of reservoir |
100 µl for initial screening, 200 µl for crystal optimization |
