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. 2017 Jun 20;73(Pt 7):423–430. doi: 10.1107/S2053230X17008500

Figure 1.

Figure 1

Purification of the MdfA–YN1074 complex and pH-dependent thermostability analyses of MdfA and the MdfA–YN1074 complex. (a) Size-exclusion chromatograms of MdfA (green) and MdfA–YN1074 (purple) on a Superdex 200 10/300 GL size-exclusion column. Inset: SDS–PAGE analysis of the main SEC peaks. (b) Thermostability of MdfA/the MdfA–YN1074 complex as a function of pH was assessed using the CPM thermal denaturation assay. Apparent T m values for MdfA and the MdfA–YN1074 complex were evaluated from the first derivative of the melting curve.