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. 2017 Jun 20;73(Pt 7):423–430. doi: 10.1107/S2053230X17008500

Table 1. MdfA production information.

Source organism E. coli
DNA source E. coli Top10
Forward primer AGGAGACTCGAGATGCAAAATAAATTAGCT
Reverse primer TTTCGGATCCATAGGTACCCCTTCGTGCGAA
Expression vector pWaldo-GFPe
Expression host E. coli C43 (DE3)
Complete amino-acid sequence of the construct produced MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNNMIQPGMLAVVEQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLAQNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLVGAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKNGRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSLMVIFLKDKQMGNSHEG