Table 1. MdfA production information.
Source organism | E. coli |
DNA source | E. coli Top10 |
Forward primer | AGGAGACTCGAGATGCAAAATAAATTAGCT |
Reverse primer | TTTCGGATCCATAGGTACCCCTTCGTGCGAA |
Expression vector | pWaldo-GFPe |
Expression host | E. coli C43 (DE3) |
Complete amino-acid sequence of the construct produced | MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNNMIQPGMLAVVEQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLAQNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLVGAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKNGRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSLMVIFLKDKQMGNSHEG |