. 2017 Jun 20;73(Pt 7):423–430. doi: 10.1107/S2053230X17008500

Table 2. Crystallization of MdfA and MdfA–YN1074.

Protein	MdfA	MdfA–YN1074	MdfA–YN1074
Method	Hanging-drop vapour diffusion	Hanging-drop vapour diffusion	Lipidic cubic phase
Temperature (K)	289	289	293
Protein concentration (mg ml⁻¹)	5	2.5	2.5
Buffer composition of protein solution	10 mM MES, 20 mM NaCl, 0.02% DDM pH 7.0	10 mM MES, 20 mM NaCl, 0.02% DDM pH 7.0	10 mM MES, 20 mM NaCl, 0.02% DDM pH 7.0
Composition of reservoir solution	100 mM Tris pH 7.5, 100 mM NaCl, 100 mM Li₂SO₄, 28–30% PEG 400	100 mM HEPES pH 7, 100 mM CdCl₂, 100 mM LiCl, 24–28% PEG 400	100 mM ADA pH 6.5, 100 mM NaCl, 100 mM Li₂SO₄, 32–36% PEG 300
Volume and ratio of drop	2 µl (1:1)	2 µl (1:1)	100 nl
Volume of reservoir	1 ml	1 ml	1 µl