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. 2017 Jun 28;73(Pt 7):431–436. doi: 10.1107/S2053230X17009025

Table 2. Crystallization.

Method Hanging-drop vapour diffusion
Temperature (K) 293
Protein concentration (mg ml−1) 20
Buffer composition of protein solution 10 mM Tris–HCl pH 8.0, 100 mM NaCl, 3 mM DTT
Composition of reservoir solution 0.01 M magnesium acetate tetrahydrate, 0.05 M sodium cacodylate trihydrate pH 6.5, 1.3 M lithium sulfate monohydrate
Volume and ratio of drop 2 µl protein solution mixed with 2 µl reservoir solution
Volume of reservoir (µl) 500