Table 3. Data collection and processing.

Values in parentheses are for the outer shell.

	Native VqsR-N	SeMet VqsR-N
Diffraction source	BL17U1, SSRF	BL17U1, SSRF
Wavelength (Å)	0.9791	0.9791
Temperature (K)	100	100
Detector	ADSC Quantum 315r CCD	ADSC Quantum 315r CCD
Crystal-to-detector distance (mm)	250	250
Rotation range per image (°)	1	1
Total rotation range (°)	180	360
Exposure time per image (s)	0.5	0.5
Space group	I222	I222
a, b, c (Å)	52.83, 135.20, 150.77	52.98, 134.78, 150.21
α, β, γ (°)	90, 90, 90	90, 90, 90
Mosaicity (°)	0.17–0.25	0.26–0.38
Resolution range (Å)	50.00–2.10 (2.16–2.10)	50.00–2.20 (2.28–2.20)
Total No. of reflections	226958	400208
No. of unique reflections	32086	27810
Completeness (%)	99.9 (100.0)	100.0 (100.0)
Multiplicity	7.1 (7.4)	14.4 (14.9)
〈I/σ(I)〉	26.04 (7.33)	56.93 (8.46)
R r.i.m. (%)	7.3 (37.5)	9.7 (49.1)
Overall B factor from Wilson plot (Å2)	34.69	37.79