Table 1. Macromolecule-production information.

Source organism	M. smegmatis mc2 155
DNA source	M. smegmatis genomic DNA
Forward primer†	GAGTCCATATGGTGAGCAAGACTGTCGAGGTCG
Reverse primer‡	TACTAGCGGCCGCTCAGCTCTGGGTGAGCTGCT
Cloning vector	pET-28a(+) modified by replacing the thrombin cleavage site with a TEV site
Expression vector	pET-28a(+) modified by replacing the thrombin cleavage site with a TEV site
Expression host	E. coli strain BL21(DE3)
Complete amino-acid sequence of the construct produced§	MGSSHHHHHHSSGENLYFQG HMVSKTVEVAASAETITSIVSDFEAYPQWNPEIKGCWILARYNDGRPSQLRLDVEIQGQSGVFITAVYYPAENQIFTMLQQGDHFTKQEQRFSIVPLGPDSTLLQVDLDVEVKLPVPGPMVKKLAGETLEHLAKALEGRVEQLTQS

^†The NdeI site is underlined.

^‡The NotI site is underlined.

^§The His-tag sequence and the cleavage site of TEV protease are underlined. The amino-acid sequence in italics is the crystallized protein amino-acid sequence.