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. 2017 Jun 30;73(Pt 7):437–442. doi: 10.1107/S2053230X17008937

Table 2. Crystallization.

Method Hanging-drop vapour diffusion
Plate type 24-well hanging-drop plate
Temperature (K) 289
Protein concentration (mg ml−1) 5
Buffer composition of protein solution 20 mM Tris–HCl pH 7.4, 150 mM NaCl, 5%(v/v) glycerol
Composition of reservoir solution 2.0 M sodium formate, 0.1 M MES pH 6.5
Volume and ratio of drop 2 µl (1:1)
Volume of reservoir (µl) 500