. Author manuscript; available in PMC: 2018 Jun 1.

Published in final edited form as: Endocr Relat Cancer. 2017 Mar 28;24(6):253–265. doi: 10.1530/ERC-16-0536

Table 1.

Histopathology of the gastrointestinal tract in control and Lgr5+ stem cell specific Pten knockout mice fed either a low fat or high-fat diet

	Low-fat diet		High-fat diet
	Control (n=8)	Pten KO (n=9)	Control (n=14)	Pten KO (n=17)
Hyperplasia, crypt epithelial (Focal)^†	0±0	0±0	0.14±0.14	0.29±0.20
Hyperplasia, crypt epithelial (Multifocal)^†	1.28±0.18^a	1.27±0.25^a	1.07±0.47^a	0.47±0.12^b
Dysplastic foci^‡	0±0	0.11±0.11	0.28±0.13	0.29±0.19
Macroadenomas^#	0±0	0±0	0±0	0±0
Carcinomas^‡	0±0	0±0	0±0	0.06±0.06
Dysplasia, Colon	0±0	0±0	0±0	0.11±0.08

Data are means±SE. Non-parametric data were analyzed by the Kruskal-Wallis non-parametric test planned contrast performed by Mann-Whitney U. A significant effect was observed for multifocal crypt hyperplasia (chi-square=10.2; P=0.017), with a significant reduction in Pten KO mice on HFD. Different letters denote a significant difference between groups, P<0.05.

Value based upon post-mortem analysis of total tumor multiplicity throughout the intestinal tract. Also shown in Fig. 2A.

^†

Value based on the pathologic severity using a 1–4 scale, with 4 being most severe.

^‡

Value indicates the number of identified dysplastic foci per section.