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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: J Immunol. 2017 May 24;199(1):271–277. doi: 10.4049/jimmunol.1700243

Figure 4. Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake.

Figure 4

(A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε-/- BMDM, were attached to IgG surfaces (20 min, 37°C) and capacitance quantified by whole cell patch-clamping. Data is reported as mean ± SEM, 15 cells/condition from two animals of each genotype. (B) BMDM expressing PKC-ε-GFP were treated with PIK93 and phagocytosis followed in real time (Movies 1 and 2). A selected frame from Movies 1 and 2 is shown with 4 targets identified (1-4) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p< 0.001 compared to No treatment

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