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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Cancer Res. 2017 May 16;77(13):3455–3466. doi: 10.1158/0008-5472.CAN-16-3240

Figure 4.

Figure 4

A cancer stem cell-like population is increased in suspension. A, CD24 staining of MDA-MB-453 (MDA453) cells following 5 days in suspension culture. B, mammosphere formation efficiency (MFE) assay of MDA453 cells placed in attached or suspended conditions for 5 days prior to plating. C, ALDEFLUOR (ALDH+) assay of SUM159PT cells placed in suspended conditions for 3 days. DEAB was used as a control to set the gate. D, MFE assay of SUM159PT cells placed in attached or suspended conditions for 3 days prior to plating. E, SUM159PT cells cultured for 3 days in suspension were sorted for ALDH and AR gene expression was measured using quantitative real-time PCR (qRT-PCR). Shown is a representative image of sorted ALDH populations. Mean ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001.