Extracellular flux analysis in TRAMP-C2, Myc-CaP and 22rv1 cells incubated with 100 μM DFP for 24 h. A, Basal ECAR measurements for treated (DFP) and untreated (Ctr) groups, averaged over 15 min experiment time. B, Average OCR time-course measurements in TRAMP-C2 cells (single, control experiment) during sequential injections of different drugs, and ranges for calculating the functional parameter, as described by the manufacturer. C, Mitochondrial functional parameters derived from OCR time-course curves, for treated (DFP) and untreated (Ctr) groups.