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. Author manuscript; available in PMC: 2017 Jul 11.
Published in final edited form as: Planta Med. 2013 Dec 5;80(1):97–104. doi: 10.1055/s-0033-1351097

Figure 2.

Figure 2

Maximum fold-induction of the ARE luciferase reporter by tBHQ, CDDO-Im, and natural compounds. AREc32 cells were seeded at 3,500 cells/well in a 384-well plate, 46 µL/well. 24 h after seeding, cells were treated with compound (0.02–30 µM) or DMSO vehicle. 24 h after treatment, luciferase activity was assessed using the Steady-Glo luciferase assay with a luminescence readout. The luminescence of each well was divided by the median luminescence of the DMSO vehicle control wells to generate the fold Nrf2 activation.