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. Author manuscript; available in PMC: 2017 Nov 30.
Published in final edited form as: Nature. 2017 May 31;546(7658):381–386. doi: 10.1038/nature22405

Figure 3.

Figure 3

ACSS2 ChIP-seq localization is linked to histone acetylation in vivo in mouse hippocampus (HPC). (a) ChIP-seq for ACSS2 and H3K9ac in mouse HPC. Track-views show ACSS2 and H3K9ac for three canonical neuronal genes involved in memory: Arc, Egr2, and Nr2f2 (chr15:74496025–74506488, chr10:66991018–67006804, and chr7:77488549–77516626, respectively). (b) Venn diagram shows in vivo HPC ACSS2 and H3K9ac peaks co-localize with the nearest gene TSS (< 1kb from peak) among all RefSeq transcripts. (c) RNA-seq expression in dorsal HPC correlates with ACSS2 binding and enrichment of H3K9 acetylation. (d) Expression profile of genes classified by way of their ACSS2 and H3K9ac enrichment state (d) Venn diagram shows the overlap between ACSS2 targeted genes (HPC), and CBP and H3K27ac enrichment for entire set of peaks (ENCODE CBP and H3K27ac ChIP-seq in mouse forebrain and cortex). (e) RNA expression heat map in HPC showing co-enrichment of ACSS2 and H3K9ac. (f) Motif analysis at ACSS2 peaks from in vivo ChIP-seq in HPC showing top enrichment of NRF1, a neuronal transcription factor.