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. Author manuscript; available in PMC: 2017 Jul 11.
Published in final edited form as: Methods Enzymol. 2016 Feb 16;573:43–65. doi: 10.1016/bs.mie.2016.01.003
18.0 μl 109 μM NCP prep 196 (final concentration 1 mg/ml)
20.8 μl 235 μM PRC1 ub module prep 1 (2.5 molar equivalents to NCP)
361 μl reconstitution buffer (10 mM Tris-Cl pH 7.6, 35 mM NaCl, 1 mM DTT, 10 μM ZnSO4)
400 μl total volume