Preparation, crystallization and structure determination of chromatin enzyme/nucleosome complexes

. Author manuscript; available in PMC: 2017 Jul 11.

Published in final edited form as: Methods Enzymol. 2016 Feb 16;573:43–65. doi: 10.1016/bs.mie.2016.01.003

361 μl	reconstitution buffer
18.0 μl	109 μM NCP prep 196
5.2 μl	235 μM PRC1 ub module prep 1
5.2 μl	obs: precipitation, then immediate clearing
5.2 μl	235 μM PRC1 ub module prep 1
5.2 μl	obs: precipitation, then immediate clearing
5.2 μl	235 μM PRC1 ub module prep 1
5.2 μl	obs: precipitation, then immediate clearing
5.2 μl	235 μM PRC1 ub module prep 1
5.2 μl	obs: precipitation, then immediate clearing