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. Author manuscript; available in PMC: 2017 Jul 11.
Published in final edited form as: Methods Enzymol. 2016 Feb 16;573:43–65. doi: 10.1016/bs.mie.2016.01.003
59.6 μl MilliQ water
121 μl 62.9 μM NCP prep 222
7.5 μl 1 M Tris-Cl pH 7.6
1.9 μl 5 M NaCl
27.6 μl 120 μM PRC1 ub module prep 3
 obs: precipitation, then immediate clearing
27.6 μl 120 μM PRC1 ub module prep 3
 obs: precipitation, then immediate clearing
27.6 μl 120 μM PRC1 ub module prep 3
 obs: precipitation, then immediate clearing
27.6 μl 120 μM PRC1 ub module prep 3
 obs: precipitation, with some cloudiness remaining
300 μl total volume