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. 2017 May 10;7(1):11–18. doi: 10.11138/mltj/2017.7.1.011

Figure 2.

Figure 2.

Cell tracking assay with CFSE CellTrace™. Tenocytes derived from tendon were stained with CFSE CellTrace™ on day 0. Fluorescence intensity was measured by FACS Calibur flow cytometer in FL1 channel at 48 and 72 h. a) Effect of 10µu/ml AA, 50µu/ml AA, 10−7M T3, 10µu/ml AA + 10−7M T3 and 50µu/ml AA +10−7M T3 on cell proliferation. Fluorescence dilution reflects cell proliferation. A representative histogram shows the highest decrease of CFSE fluorescence adding 50µu/ml AA +10−7M T3 versus control untreated cells. The graph represents the quantification of CFSE median fluorescence index of three independent experiments. b) Histograms show the mean (± SD) of the successive generations of one experiment out of three. Standard deviations are shown.