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. 2017 Jul 6;10:3313–3327. doi: 10.2147/OTT.S140492

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© 2017 Woitok et al. This work is published and licensed by Dove Medical Press Limited

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Specific ex vivo binding studies.

Notes: Tumor tissue biopsies from breast cancer patients were used to test the ex vivo binding of two different ADCs. Representative images show FFPE tumor sections stained with new fuchsin and counterstained with hematoxylin and eosin (objective =40×, scale bars =50 µm). The specific binding of scFv-SNAP-AURIF is indicated by red-stained EGFR⁺ clusters, and areas of interest are marked with arrows. A mock-SNAP-AURIF construct was used as a control. Further controls were prepared by staining with the detection antibody only (anti-SNAP antibody M2D11 and GAM^AP).

Abbreviations: ADC, antibody–drug conjugate; AURIF, auristatin F; EGFR, epidermal growth factor receptor; FFPE, formalin-fixed paraffin-embedded; scFv, single-chain fragment variable.