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Western blot and NTA validation of exosomal samples.
Notes: Western blots of (A) breast milk exosomes (60 μg/lane) [(A1) clathrin and (A2) CD9] and (B, C) plasma exosomes (25 μg/lane) [(B1) CD9, (B2) CD63], (C) TIM-4. Arrows indicate proteins of interest. (D, E) NTA-generated size and concentration plots for (D) human plasma- and (E) human breast milk-derived exosomes.
Abbreviations: NTA, nanoparticle tracking analysis; TIM, T cell immunoglobulin and mucin.
