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. 2017 Jul 4;10:3289–3297. doi: 10.2147/OTT.S138123

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© 2017 Chang et al. This work is published and licensed by Dove Medical Press Limited

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NAC effect on ROS generation of sinuleptolide-treated oral cancer cells and oral normal cells.

Notes: NAC pretreatment condition was 4 mM for 1 h. No NAC pretreatment was kept in culture medium for 1 h. Oral cancer cells (Ca9-22) were incubated with 0, 12, and 24 µg/mL of sinuleptolide for 6 h. (A and B) Typical ROS patterns of sinuleptolide treated Ca9-22 and HGF-1 cells. The right side of each flow cytometry panel indicates the ROS positive (+%) region. (C and D) Statistics of relative mean intensity of ROS pattern (in A and B). Data, mean ± SD (n=3). **P-value of <0.002 for the significance between data with or without NAC pretreatment.

Abbreviations: NAC, N-acetylcysteine; ROS, reactive oxygen species.