Figure 5.

NAC effect on γH2AX-based DNA damage in sinuleptolide-treated oral cancer and oral normal cells.

Notes: NAC pretreatment condition was 4 mM for 1 h. No NAC pretreatment was kept in culture medium for 1 h. Oral cancer cells (Ca9-22) were incubated with 0, 6, 12, and 24 µg/mL of sinuleptolide for 24 h. (A and B) Typical γH2AX pattern of sinuleptolide-treated Ca9-22 and HGF-1 cells. Dashed lines of each flow cytometry panel indicate the γH2AX positive (+%) regions. (C and D) Statistics of mean intensity of γH2AX-based DNA damage pattern (in A and B). Data, mean ± SD (n=3). **P-value of <0.002 for the significance between data with or without NAC pretreatment.

Abbreviations: NAC, N-acetylcysteine; PI, propidium iodide.