A. Detection of antigen-specific CD4+ T cells by CD154 and CD69 surface markers and expression of CD38 on memory cells in a T1D subject.
B. ICOS expression on different CD154+CD69+CD38+/- subsets in a T1D subject.
C. ICOS expression on different CD154+CD69+CD38+/- subsets in 5 T1D subjects.
D. Comparison of total βCA-specific CD4+ T cells between T1D subjects and healthy controls.
E. Comparison of naïve (CD4+CD45RO-) βCA-specific T cells between T1D subjects and healthy controls.
F. Comparison of memory (CD4+CD45RO+) βCA-specific T cells between T1D subjects and healthy controls.
G. Comparison of immune active (CD4+CD45RO+CD38+) memory βCA specific T cells between T1D subjects and healthy controls.
Data were compared using two-tail unpaired Student’s t test.