Table 1.
Antigenic epitopes identified in 4 T1D subjects
| T1D Subject | Protein | Peptide # | Peptide Sequence |
|---|---|---|---|
|
| |||
| K787T1D | Insulin | PPI78-90K88S | QPLALEGSLQSRG |
|
| |||
| GAD65 | GADp15 | DVMNILLQYVVKSFDRSTKV | |
| GADp45 | ICKKYKIWMHVDAAWGGGLL | ||
| GADp47 | GGLLMSRKHKWKLSGVERAN | ||
| GADp48 | HKWKLSGVERANSVTWNPHK | ||
| GADp55 | YDLSYDTGDKALQCGRHVDV | ||
|
| |||
| ZnT8 | ZnT8p28 | ILKDFSILLMEGVPKSLNYS | |
| ZnT8p36 | VRREIAKALSKSFTMHSLTI | ||
|
| |||
| IGRP | IGp39 | QLYHFLQIPTHEEHLFYVLS | |
|
| |||
| ChgA | NEI | ||
|
| |||
| K276T1D | Insulin | PPI78-90K88S | QPLALEGSLQSRG |
|
| |||
| GAD65 | GADp23 | RYFNQLSTGLDMVGLAADWL | |
|
| |||
| ZnT8 | ZnT8p28 | ILKDFSILLMEGVPKSLNYS | |
|
| |||
| IGRP | IGp31 | KWCANPDWIHIDTTPFAGLV | |
|
| |||
| ChgA | NEI | ||
|
| |||
| K743T1D | Insulin | PPI78-90K88S | QPLALEGSLQSRG |
|
| |||
| GAD65 | GADp28 | EYVTLKKMREIIGWPGGSGD | |
| GADp29 | REIIGWPGGSGDGIFSPGGA | ||
| GADp32 | YAMMIARFKMFPEVKEKGMA | ||
| GADp34 | KGMAALPRLIAFTSEHSHFS | ||
| GADp35 | LIAFTSEHSHFSLKKGAAAL | ||
|
| |||
| ZnT8 | ZnT8p28 | ILKDFSILLMEGVPKSLNYS | |
|
| |||
| IGRP | IGp23 | HQVILGVIGGMLVAEAFEHT | |
|
| |||
| ChgA | NEI | ||
|
| |||
| K877T1D # | GAD65 | GADp70 | KVNFFRMVISNPAATHQDID |
|
| |||
| K877T1D* | Insulin | PPI78-90K88S | QPLALEGSLQSRG |
|
| |||
| GAD65 | GADp10 | CACDQKPCSCSKVDVNYAFL | |
| GADp11 | SCSKVDVNYAFLHATDLLPA | ||
|
| |||
| ZnT8 | ZnT8p28 | ILKDFSILLMEGVPKSLNYS | |
|
| |||
| IGRP | NEI | ||
|
| |||
| ChgA | NEI | ||
NEI: No Epitope Identified
#
For subject K877T1D, only four CD38+ memory T cells were obtained for oligoclonal expansion and epitope mapping.
*
Since only four CD38+ memory T cells were obtained from subject K877T1D for oligoclonal expansion and epitope mapping, oligoclones expanded from resting memory CD4 T cells were used to identify the epitopes for this subject.